Spectroscopy is a powerful tool in the fields of physics and chemistry for studying the interaction of optical radiation with matter providing important and detailed information about the structure and dynamics of the system under study. Spectroscopic techniques can be classified according to the nature of the interaction between radiation and matter (absorbance, transmittance, reflectance, scattering and emission) or the spectral region of the radiation used for illumination/excitation (ultraviolet, visible and infrared). Quite often, the spectral region of the radiation used defines the name of the spectroscopic technique such as UV-Vis-NIR or IR absorption spectroscopy.
Absorbance / Transmittance
A technique that measures how much light is absorbed/transmitted by a transparent and finite length sample over the UV-Vis-NIR range to gain information about their spectral properties.
A technique that measures how much light is reflected over the UV-Vis-NIR range in a specular or diffuse manner from a non-transparent sample surface.
A technique that measures the amount of light that is emitted by a fluorophore upon the absorption of UV-Vis light.