Fluorescence spectroscopy is an extremely useful and ultrasensitive analytical technique to analyze the fluorescence emission of certain molecules that arises from the excitation with ultraviolet or visible light. The fluorescent properties of these molecules can be detected using spectrofluorometers, a spectroscopic instrument designed with a right-angle configuration to measure the various parameters of fluorescence, including its intensity and wavelength distribution over the optical range.
What is Fluorescence?
Typical setups for Fluorescence Measurements
Sensitivity is a key factor when performing fluorescence measurements. To achieve high sensitivity in our compact and modular spectrometers, it is important to combine, on the emission detection side, the high sensitivity of a back-thinned CCD detector (choose the thermoelectrically cooled version for getting the ultimate sensitivity), which has a maximum quantum efficiency of around 80% (at the peak), with collecting lens and large entrance slits (200 µm). On the excitation side, a high power light source is crucial. Sarspec has two great light sources capable of delivering a stable and high power excitation: LEDs light source (LS-LED) for narrow bandpass excitation over the UV-Vis-NIR wavelength range and Xenon Flashlamp light source (LS-XeF) when excitation needs to be performed with power in deep-UV.