SPEC SENSE+ / SPEC SENSE+ UV/Vis
• Wavelength range: 200-880 nm
• Quantum Efficiency of 78% at 600 nm
• Back thinned 2048 pixels CCD array detector
• 600 grooves/mm grating blazed at 300 nm
• Collecting Lens included
• LightScan software included
The SPEC SENSE+ UV/Vis is configured with a 600 grooves/mm grating blazed at 300 nm, 200 µm slit and collecting lens allowing high sensitivity measurements in a wavelength range of 200-880 nm.
This flexible and highly sensitive spectrometer is ideal for low light emitting sources, but also for fluorescence and chemiluminescence of biological samples.
The extreme sensitivity of this spectrometer is confirmed by the results obtained with Tryptophan. This coumpound has a low quantum yield of 0.13 which makes it ideal for sensitivity testing. The minimum concentration tested was 0.416 micromolar (uM) and the curve was built using 360 nm as the emission peak. This configuration is available as pre-configured solution Fluorescence SPEC Pack XeF. The results can be found in the graphics below:
| Wavelength range | 200-880 nm |
| Slit | 200 µm |
| Grating | 600 lines @ 300 nm |
| Resolution | 5.4 nm |
| Detector | 2048 pixels CCD array back thinned Hamamatsu |
| Pixel width x Height | 14 x 1000 µm |
| Optical design | Symmetrical Czerny-Turner |
| Order sorting filters | Yes |
| Longpass filters | - |
| Focal length | 50 mm |
| Dark noise (RMS) | 19 |
| Dynamic range | 3450 |
| Signal/Noise | 509 |
| Linearity | 99.99 % |
| Integration time | 1 ms - 2.8 s |
| QE (%) @ peak | 78% |
| Peak wavelength | 600 nm |
| Pixel well depth | 2000000 |
| PRNU | 3% |
| A/D converter | 16 bits |
| Interface | Mini USB 2.0 |
| Trigger | In/Out |
| Trigger delay | Out |
| Fiber connector | SMA 905 |
| Weight | 650 g |
| Dimensions | 119 (L) x 90 (D) x 60 (H) |
| Collecting Lens | Included |
The image below shows the spectra of a Light Source (LS-DW) from Sarspec, obtained with all four configurations from the SPEC SENSE+ family.
