SPEC SENSE+ / SPEC SENSE+ UV/Vis

​• Wavelength range: 200-880 nm
​• Quantum Efficiency of 78% at 600 nm
​• Back thinned 2048 pixels CCD array detector
​• 600 grooves/mm grating blazed at 300 nm
​• Collecting Lens included
​• LightScan software included

The SPEC SENSE+ UV/Vis is configured with a 600 grooves/mm grating blazed at 300 nm, 200 µm slit and collecting lens allowing high sensitivity measurements in a wavelength range of 200-880 nm. 

This flexible and highly sensitive spectrometer is ideal for low light emitting sources, but also for fluorescence and chemiluminescence of biological samples. 

The extreme sensitivity of this spectrometer is confirmed by the results obtained with Tryptophan. This coumpound has a low quantum yield of 0.13 which makes it ideal for sensitivity testing. The minimum concentration tested was 0.416 micromolar (uM) and the curve was built using 360 nm as the emission peak. This configuration is available as pre-configured solution Fluorescence SPEC Pack XeF. The results can be found in the graphics below:

 

 

Wavelength range 200-880 nm
Slit 200 µm
Grating 600 lines @ 300 nm
Resolution 5.4 nm
Detector 2048 pixels CCD array back thinned Hamamatsu
Pixel width x Height 14 x 1000 µm
Optical design Symmetrical Czerny-Turner
Order sorting filters Yes
Longpass filters -
Focal length 50 mm
Dark noise (RMS) 19
Dynamic range 3450
Signal/Noise 509
Linearity 99.99 %
Integration time 1 ms - 2.8 s
QE (%) @ peak 78%
Peak wavelength 600 nm
Pixel well depth 2000000
PRNU 3%
A/D converter 16 bits
Interface Mini USB 2.0
Trigger In/Out
Trigger delay Out
Fiber connector SMA 905
Weight 650 g
Dimensions 119 (L) x 90 (D) x 60 (H)
Collecting Lens Included

 

The image below shows the spectra of a Light Source (LS-DW) from Sarspec, obtained with all four configurations from the SPEC SENSE+ family.

 

                   

Description Reference
SPEC SENSE+ UV/Vis SP-SENSE-UV-Vis

 

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