SPEC SENSE+ / SPEC SENSE+ FL
• The best option for High Sensitivity Fluorescence
• Wavelength range: 300-1000 nm
• Quantum Efficiency of 75% at 500 nm
• 600 grooves/mm grating blazed at 500 nm
• Order sorting filters, collecting lens included
• External trigger (IN/OUT) for easy synchronization
The SPEC SENSE+ FL was specially designed for fluorescence measurements granting the best sensitivity for experiments, even in compounds with low quantum yield. The Quantum Efficiency (75% at 500 nm) of the back thinned CCD combined with the large pixel area, 200 µm slit, collecting lens and a 600 grooves/mm grating make these spectrometers the best option within it's wavelength range.
With special focus on Fluorescence, Phosphorescence, Chemi and Bioluminescence present in biomolecules such as amino acids, proteins, amines, lipids, porphyrins, steroids, vitamins, etc., the traditional application areas for the SPEC SENSE+ FL are Biology, Biophysics, Chemistry, Pharmaceutical and Medical research and quality control.
The application below takes us to femtomolar concentration of Eosin, using the SPEC SENSE+ FL, Light Lource LED (LS-LED) with a 495nm LED, multipurpose cuvette holder (MCH) and 1000 µm core diameter optical fibers (2 units). This configuration is available as a pre-configured solution FLUORESCENCE SPEC PACK CUV.
|Wavelength range||300-1000 nm|
|Grating||600 lines @ 500 nm|
|Detector||2048 pixels CCD array back thinned Hamamatsu|
|Pixel width x Height||14 x 1000 µm|
|Optical design||Symmetrical Czerny-Turner|
|Order sorting filters||Yes|
|Focal length||50 mm|
|Dark noise (RMS)||19|
|Integration time||1 ms - 2.8 s|
|QE (%) @ peak||78%|
|Peak wavelength||600 nm|
|Pixel well depth||2000000|
|A/D converter||16 bits|
|Interface||Mini USB 2.0|
|Fiber connector||SMA 905|
|Dimensions||119 (L) x 90 (D) x 60 (H)|
The image below shows the spectra of a Light Source (LS-DW) from Sarspec, obtained with all four configurations from the SPEC SENSE+ family.
|SPEC SENSE+ FL||SP-SENSE-FL|