FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK XeF
When high power excitation in the UV is required the light source is normally quite expensive. An affordable solution for users that want to work with UV excitation and obtain good results in Fluorescence is to use the Xenon Flashlamp Light Source LS-XeF from Sarspec. With it, it's possible to obtain great fluorescence results with high flexibility.
The full configuration includes the high sensitivity spectrometer SPEC SENSE+ UV/Vis with a back-thinned CCD array with 2048 elements and great quantum efficiency in the UV Range, optical fibers with 1000 um core diameter (50 cm), the multipurpose cuvette holder CH-MP with filter slots, large lens and signal enhancing mirrors (CH-MP-EM).
Tryptophan was tested with this configuration. Tryptophan is an amino acid with a really low quantum yield of 0.13 and is an essential amino acid in the human diet. The configuration used to test it was the Fluorescence SPEC PACK XeF. Emission wavelength considered was 360 nm. Three solutions were prepared in HEPES with concentrations of 0.416, 2.08 and 10.4 MICROMOLAR and the results are shown below:
The pre-configured solution Fluorescence SPEC PACK XeF includes the following components:
Pre-configured solution for Fluorescence using cuvettes including:
SP-SENSE-UV-Vis; LS-XeF; CH-MP, CH-MP-EM and OF-UV-1000-50 (2x)