FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK CUV
This is the most sensitive configuration available, mainly due to the sensitivity of the back thinned CCD array with high quantum efficiency (up to 78%) together with 200 µm slit, and collecting lens for the detector.
Also, the high power LEDs from the flexible LS-LED Light Source - where many different excitation wavelengths can be used by simply changing the LED slide - contributes greatly for the final sensitivity. In this configuration the sample holder takes an important role on the sensitivity and flexibility of the solution. Sarspec’s Multipurpose Cuvette Holder uses 12.7 mm diameter lens for large sample area illumination/collection and reflecting mirrors to employ all radiation available and supports a wide range of filters easily interchangeable.
It's important to understand the expected detection limit in Fluorescence measurements. Each compound has single behaviour and quantum yield. Eosin Y is a common dye used in molecular biology applications with quantum yield of 0.67. Four solutions were prepared in ethanol with concentrations of 189, 347, 625 and 833 femtomolar and the emission wavelength considered was 544 nm.
The instrumentation used was a Fluorescence SPEC PACK CUV from Sarspec configured with a 495 nm high power LED. The spectra and linearity are below:
The pre-configured solution Fluorescence SPEC PACK CUV includes the following components:
Pre-configured solution for Fluorescence using cuvettes including:
SP-SENSE-FL; LS-LED (including one LED in Visible range); CH-MP, CH-MP-EM and OF-UV-1000-50 (2x)